While trolling through the NHTSA website, because I will do anything to procrastinate, I found an article which may explain some of the difficulty people have had with pairing aftermarket TPMS sensors:
https://static.nhtsa.gov/odi/tsbs/2025/MC-11020661-0001.pdf
It appears the GX and UX have a new generation of TPMS sensors which send information via a new, faster radio signaling protocol, which transmits information in significantly shorter bursts than before - .3ms of transmission every 30ms, or 30ms of transmission every 1 second if the vehicle is not being driven, according to the doc.
I don't know if this protocol is compatible with previous generation TPMS transmissions or if the GX will accept previous generation TPMS signals. But member jpto does
claim to have paired aftermarket BH Sens TPMS sensors, so that's at least one point of information.
Given this notice only applies to 24-25 GX550s and 25 UX300Hs, I would guess the aftermarket hasn't had time to update its line of non-programmable sensors or their database of programmable protocols yet. It's possible this is why aftermarket TPMS sensors are so difficult to reliably pair with the GX.
Interestingly, the 2025 Land Cruiser 250 does not seem to have a matching TSB logged with NHTSA, so we may be the first guinea pigs to have these next generation TPMS sensors.